Are Solid Particles Ready for Prime-Time Proteomics?

ABSTRACT: We evaluate the performance of nonporous C-18 stationary phases in high-speed proteomics workflows. We employed two commercially available sub-2 μm nonporous particle (NPP) materials, ODS-IIIE (1.5 μm) and SOLAD (1.0 μm), to fabricate analytical columns using 150 μm internal diameter (i.d.) fused silica capillaries to ensure compatibility with nano-UHPLC and nano-HPLC pressure regimes. Using "long" NPP columns (15-25 cm) connected to a conventional nano-UHPLC, we found that both materials supported efficient peptide separations within the flow rate and pressure ranges typical of nano-UHPLC systems. Shorter columns, used with the 10- and 16 min Whisper Zoom 120 and Zoom 80 methods on the Evosep One HPLC, demonstrated competitive separation performance compared to columns packed with fully p

SUBMITTER: Kitano ES 

PROVIDER: S-EPMC12409696 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature