Enzymatic Synthesis of an Undecorated Capsular Polysaccharide from <i>Campylobacter jejuni</i>.

ABSTRACT: The exterior surface of the human pathogen Campylobacter jejuni is coated with a capsular polysaccharide (CPS) that helps protect it from the host immune system. In C. jejuni NCTC 11168 the repeating linear polysaccharide is composed of d-ribose, N-acetyl-d-galactosamine and d-glucuronic acid that is further amidated with either ethanolamine or serinol. The CPS is also decorated with d-glycero-l-gluco-heptose and methyl phosphoramidate. We have now shown that the polymerization of the undecorated CPS requires the sequential activity of six unique enzymes that must act in concert with one another. The catalytic activity of these six enzymes enabled a robust synthetic strategy to be developed to facilitate the assembly and isolation of specific oligosacchar

SUBMITTER: Xiang DF 

PROVIDER: S-EPMC12409886 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature