Comparative Evaluation of Toxicity End Points in Unicellular Bioassays for Environmental Pollutant Assessment.

ABSTRACT: Aquatic environments contain mixtures of contaminants, of which many cannot be detected through conventional chemical monitoring. Effect-directed analysis (EDA) uses bioassays to detect bioactive compounds and relies on sensitive small volume assays. The aim of this study was to evaluate the sensitivity of three unicellular species (Raphidocelis subcapitata, Escherichia coli, and Saccharomyces cerevisiae) and five vertebrate cell lines to 21 chemicals with diverse modes of action. The algae assay exhibited the highest sensitivity, detecting toxicity in >80% of the chemicals, while yeast was the least responsive. Subsequently, 279 wastewater samples were screened using algae and three cell lines. Algae detected toxicity in >92% of the samples, while the cell line viabil

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12409895 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature