Single amino acid substitution at position 614 in SARS-CoV-2 Spike Protein alters viral assembly and infectivity.

ABSTRACT: The emergence of SARS-CoV-2 has posed a substantial global public - health threat and has led to the emergence of diverse variant strains. A prevalent mutation, D614G, is commonly detected in the spike glycoprotein (S) of successive SARS- CoV-2 variants, which enhances viral infectivity. Here, the objective was to examine the influence of mutations on the synthesis and processing of the S protein, virus assembly, and infectivity. This was achieved by artificially substituting the aspartic acid at position 614 of the S protein with 19 distinct amino acids, including glycine, via codon modification. Pseudoviruses and virus-like particles were employed as models for this investigation. The results demonstrated that the expression characteristics of the modified S proteins diverged from those

SUBMITTER: Shi X 

PROVIDER: S-EPMC12410178 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature