Proteomic insights into collagen remodeling of the calipash in Chinese soft-shelled turtle (<i>Pelodiscus sinensis</i>) under <i>Aeromonas hydrophila</i> challenge: Implications for nutritional quality and oxidative stress response.

ABSTRACT: The calipash, a collagen-rich tissue in Pelodiscus sinensis, undergoes structural degradation during Aeromonas hydrophila infection, compromising its economic value. This study investigates the underlying collagen alterations. Turtles were challenged with A. hydrophila, and samples were collected at 0 h, 6 h, 1d, 3d, 6d, and 10d post-infection. Results showed progressive collagen degradation, peaking at 10d. Early infection increased collagen content, type I collagen proportion, and collagen-related gene expression, but these declined over time. Essential amino acids decreased, while non-essential amino acids (e.g., glycine, proline) increased. iTRAQ proteomics identified 517 differentially expressed proteins (227 up-, 290 down-regulated), including collagen metabolism

SUBMITTER: Luo J 

PROVIDER: S-EPMC12410406 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature