Enhancing diagnostic efficiency of pyrazinamide resistance in <i>Mycobacterium tuberculosis</i> via modified MGIT assay and genotypic correlation.

ABSTRACT: Pyrazinamide (PZA) plays a crucial role in the treatment of both active and latent tuberculosis, particularly in regimens designed to treat drug-resistant TB. However, diagnosing resistance to PZA poses challenges for managing TB, highlighting the need for accurate detection methods. This study aims to address the challenges in detecting PZA resistance by modifying the standard MGIT960 PZA drug susceptibility testing method by optimizing the inoculum dilution. Briefly, three MGIT DST versions were evaluated: the standard method, the reduced inoculum (RI) method employing a 1:20 inoculum dilution and the sparse dilution (SD) method using a 1:50 dilution of the inoculum for growth control tube, while the undiluted MGIT positive culture was used for the PZA test tube. The SD MGIT DST approach

SUBMITTER: Rajendran A 

PROVIDER: S-EPMC12410468 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature