Rational design of respiratory syncytial virus dimeric F-subunit vaccines in protein and mRNA forms.

ABSTRACT:

Background

Respiratory syncytial virus (RSV) poses a significant public health threat, particularly to children and the elderly. Two protein-based vaccines and one mRNA vaccine have been approved, all targeting the prefusion conformation of the fusion (F) trimer. However, it has been reported that the epitope activity of the F protein gradually declines during storage, resulting in a reduction of the vaccines' immunogenicity.

Methods

In this study, we engineered a redesigned pre-F-based antigen, monomer A, derive from the RSV subtype A F protein, aiming to preserve immunodominant pre-F-specific epitopes while eliminating sub-potent ones. Following this design principle, we constructed a series of single-chain (sc) dimers and selected the one, scDimer AA, with the highest exp

SUBMITTER: Li J 

PROVIDER: S-EPMC12410573 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature