Determining minimal inhibitory concentrations and antibiotic susceptibility for Enterobacterales by flow cytometry using reactive oxygen species as a marker.

ABSTRACT: Early appropriate antibiotic treatment is vital in reducing patient mortality. However, current antimicrobial susceptibility testing (AST) requires 16-24 hours of incubation, delaying appropriate antibiotic treatment. Flow cytometry (FCM) is a rapid method in assessing fluorescence (such as fluorophores for ROS) at single-cell resolution. Reactive oxygen species (ROS) are oxygen-containing molecules, which are inducible by antibiotics and potentially bactericidal. We asked if FCM measurements of ROS in antibiotic-treated bacteria could be used in determining antibiotic MIC as an alternative to conventional AST. This study aims to develop and evaluate the feasibility of a FCM assay to determine antibiotic susceptibility accurately with a short turn-around time. MICs of amikacins, aztreonams

SUBMITTER: Yeo JH 

PROVIDER: S-EPMC12410812 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature