A capture enzyme-linked immunosorbent assay for detection of mosquito salivary protein-specific immunoglobulin E.

ABSTRACT: Mosquito saliva contains numerous distinct mosquito salivary proteins (MSPs) that mediate mosquito-host interactions. Repeated mosquito exposure can trigger allergic reactions, with MSP-specific IgE playing a central role. Current enzyme-linked immunosorbent assay (ELISA) and immunoblotting methods for detecting MSP-specific IgE suffer from interference by much more abundant MSP-specific IgG, leading to low sensitivity. Here, we developed a capture ELISA to overcome these limitations. We compared the performance of this capture ELISA with the conventional indirect ELISA in detecting MSP-specific IgE titers in sera from both repeatedly exposed mice and human volunteers. The results demonstrated that, compared to the indirect ELISA, the novel capture ELISA exhibited significantly superior se

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12410881 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature