Functionalized Multi-Walled Carbon Nanotube Enhanced Myogenic Differentiation for Aligned Topography-Induced Skeletal Muscle Engineering.

ABSTRACT: Skeletal muscle engineering utilizing bio-activators and myogenic cells to regenerate tissues for volumetric muscle loss offers a promising alternative to tissue grafts. Modified biointerfaces with aligned micro-scale topography and electroconductivity are critical for directing cellular behavior toward functional muscle constructs. This study modified polydimethylsiloxane (PDMS) with aligned surface topography and functionalized multi-walled carbon nanotubes (fCNTs), creating a conductive scaffold (0.11 µScm^-1 vs original 0.51 nScm^-1) with regulated hydrophilicity (76 ± 2° vs original 50 ± 10° in water contact angle) and enhanced protein absorption. The fCNT-wrinkled surfaces maintained >90% cell viability while promoting aligned myotube formation. Specifically, fCNT

SUBMITTER: Feng T 

PROVIDER: S-EPMC12410905 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature