Hydrogenase Mediated Biosynthesis of Catalytically Active Cu Nanoparticles.

ABSTRACT: Shewanella oneidensis MR-1 can biosynthesize cell-supported Cu-nanoparticles (CuNPs), via the bioreduction of Cu(II)_(aq), with excellent catalytic activity for click chemistry reactions. However, enzymatic mechanisms underpinning Cu(II) bioreduction were unclear. Here, the oxidation of hydrogen as electron donor was essential for Cu(II) bioreduction by S. oneidensis and hydrogenase deletion mutants were used to demonstrate the critical role of the periplasmic [NiFe] hydrogenase, HyaB. Wild type (WT) cultured cells coupled hydrogen oxidation to biosynthesis of Cu(0)/Cu(I)-NPs within the periplasm (identified using XRD and TEM with SAED, EDS, EELS); ΔhyaB mutants did not produce CuNPs. Biosynthesized CuNPs were catalytically active for the cycloaddition of methyl azidoacetate and

SUBMITTER: Byrd N 

PROVIDER: S-EPMC12410911 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature