4-Octyl Itaconate ameliorates diesel exhaust particle-induced oxidative stress in nasal epithelial cells.

ABSTRACT:

Background and objective

Particulate matters such as diesel exhaust particles induce oxidative stress in cells and thereby have a negative impact on health. The aim of this study was to test whether the membrane-permeable, anti-inflammatory metabolite 4-Octyl Itaconate can counteract the oxidative stress induced by diesel exhaust particles and to analyze the downstream-regulated pathways both in human nasal epithelial cells and PBMCs.

Methods

Human nasal epithelial cells were cultured from nasal swabs, and the response of the cells to diesel exhaust particles either alone or in combination with 4-Octyl Itaconatee was investigated using RNA sequencing, qPCR, and cytokine measurement. The presence of reactive oxygen species in the cells was analyzed using CellROX staining and

SUBMITTER: Steppuhn H 

PROVIDER: S-EPMC12411186 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature