Improved longevity and <i>in vivo</i> performance of neurotransmitter detection using 30 µm cone-shaped carbon fiber microelectrode.

ABSTRACT: Fast Scan Cyclic Voltammetry (FSCV) is a widely used electrochemical technique to detect rapid extracellular dopamine transients in vivo. It employs carbon fiber microelectrodes (CFMEs), but conventional 7 µm diameter CFMEs often suffer from limited mechanical durability and reduced lifespan, hindering their use in chronic monitoring. To improve mechanical robustness and long-term functionality, we fabricated 30 µm diameter CFMEs and modified their geometry via electrochemical etching to form cone-shaped tips. We compared the in vitro and in vivo performance of 7 µm, 30 µm bare, and 30 µm cone-shaped CFMEs using FSCV. Electrode longevity was assessed, and biocompatibility was evaluated via immunofluorescence analysis of brain tissue. In vitro, the 30 µm bare CFMEs show

SUBMITTER: Kwon H 

PROVIDER: S-EPMC12411426 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature