Exploring the biological functions and immune regulatory roles of IRAK3, TNFRSF1A, CX3CR1, and JUNB in T2DM combined with MAFLD: integrated bioinformatics and single-cell analysis.

ABSTRACT:

Objective

Diabetes mellitus combined with nonalcoholic fatty liver disease is a prevalent and intricate metabolic disorder that presents a significant global health challenge, imposing economic and emotional burdens on society and families. An in-depth understanding of the disease pathogenesis is crucial for enhancing diagnostic and therapeutic efficacy. Therefore, the study aims to identify and validate autophagy-related diagnostic biomarkers associated with T2DM-associated MAFLD, investigate regulatory mechanisms in disease progression, and explore cellular diversity within the same tissue using single-cell sequencing data.

Methods

This study utilized four datasets retrieved from the Gene Expression Omnibus (GEO) database: GSE15653, GSE89632, GSE24807 and GSE23343. The ana

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12411428 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature