A correlation-based tool for quantifying membrane periodic skeleton associated periodicity.

ABSTRACT:

Introduction

The advent of super-resolution microscopy revealed the membrane-associated periodic skeleton (MPS), a specialized neuronal cytoskeletal structure composed of actin rings spaced 190 nm apart by two spectrin dimers. While numerous ion channels, cell adhesion molecules, and signaling proteins have been shown to associate with the MPS, tools for accurate and unbiased quantification of their periodic localization remain scarce.

Methods

We developed Napari-WaveBreaker (https://github.com/SamKVs/napari-k2-WaveBreaker), an open-source plugin for the Napari image viewer. The tool quantifies MPS periodicity using autocorrelation and assesses periodic co-distribution between targets using cross-correlation. Performance was evaluated using both simulated datasets and STED m

SUBMITTER: Vanspauwen SK 

PROVIDER: S-EPMC12411523 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature