Isolation and characterization of differentially expressed genes in the mycelium and fruit body of Tuber borchii.

ABSTRACT: The transition from vegetative mycelium to fruit body in truffles requires differentiation processes which lead to edible fruit bodies (ascomata) consisting of different cell and tissue types. The identification of genes differentially expressed during these developmental processes can contribute greatly to a better understanding of truffle morphogenesis. A cDNA library was constructed from vegetative mycelium RNAs of the white truffle Tuber borchii, and 214 cDNAs were sequenced. Up to 58% of the expressed sequence tags corresponded to known genes. The majority of the identified sequences represented housekeeping proteins, i.e., proteins involved in gene or protein expression, cell wall formation, primary and secondary metabolism, and signaling pathways. We screened 171 arrayed cDNAs by us

SUBMITTER: Lacourt I 

PROVIDER: S-EPMC124117 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature