Mechanisms of the Ershiwuwei Guijiu Pill in Treating Postmenopausal Osteoporosis Based on Network Analysis and Experimental Validation.

ABSTRACT: BackgroundThe Tibetan medicine Ershiwuwei Guijiu Pill (EWGP), a classic Tibetan medicine prescription for the treatment of postmenopausal osteoporosis (PMOP) in the Qinghai-Tibet region, has attracted extensive attention due to its curative effects on gynecological diseases. However, its chemical ingredients and molecular mechanism are still unclear.Aim of the studyTo analyze the chemical constituents and effective serum chemical metabolites of EWGP and to explore the molecular mechanism of EWGP in treating PMOP through network analysis and experimental validation.MethodsThe ethanol extract of EWGP and its drug-containing serum were detected by liquid chromatography-mass spectrometry (LC-MS), and the chemical constituents were analyzed and identified. SwissTarget prediction was used to pre

SUBMITTER: Duan F 

PROVIDER: S-EPMC12411713 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature