The pivotal role of immune functional assays in deciphering immune function alterations.

ABSTRACT: Growing evidence suggests that conventional immunomonitoring alone may not be sufficient to fully capture the complexity of immune dysfunctions. Immune functional assays (IFAs) have therefore emerged as valuable complementary tools, offering functional insights that extend beyond traditional phenotypic or quantitative approaches. Nevertheless, although in vitro stimulation represents a central component of IFAs, its specific contribution has never been rigorously evaluated, raising the critical question of whether this step is truly essential for detecting clinically relevant immune dysfunctions. To address this question, the present study compared gene expression levels (Nanostring) obtained from samples stimulated (TruCulture) or unstimulated (PaxGene) using the same analytical pipeline,

SUBMITTER: Debombourg M 

PROVIDER: S-EPMC12411760 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature