Haplotype-resolved genomes of <i>Phlebopus portentosus</i> reveal nuclear differentiation, TE-mediated variation, and saprotrophic potential.

ABSTRACT: Phlebopus portentosus is a widely consumed edible mushroom and the only Boletales species currently cultivated on an industrial scale. Despite its economic importance, its trophic strategy and genomic adaptations remain elusive. Here, we presented high-quality, chromosome-level genome assemblies for two sexually compatible monokaryons (PP78 and PP85) of P. portentosus. Comparative genomic analysis revealed a genome size difference of 1.17 Mb (30.87 vs. 32.04 Mb), primarily attributed to transposable element (TE) expansion in strain PP85. Genome structural variations were largely driven by TEs, particularly LTR retrotransposons. DNA transposons were also involved in structural rearrangement of secondary metabolite biosynthetic gene clusters, impacting their organization

SUBMITTER: Meng G 

PROVIDER: S-EPMC12411881 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature