Evaluation of [¹⁸F]F-CNBI and [¹⁸F]F-CNPI PET Probes in GSK-3β Transgenic Mice.

ABSTRACT: The present study explores the noninvasive positron emission tomography (PET) imaging for the detection of GSK-3β overexpression using [¹⁸F]F-CNPI and [¹⁸F]F-CNBI in a GSK-3β overexpressing transgenic mouse model. Herein, we validated GSK-3β overexpression in different brain regions by genotyping and Western blot. PET scans and ex vivo biodistribution were performed in normal mice and early stage and late-stage GSK-3β transgenic mice with and without the P-gp inhibitor, Tariquidar. Both probes are found in the substrate of the P-gp transporter. [¹⁸F]F-CNPI showed significantly higher brain uptake in normal and GSK-3β overexpressing mice with the P-gp inhibitor compared to those without the P-gp inhibitor. The uptake of [¹⁸F]F-CNBI was lower in al

SUBMITTER: Kethamreddy M 

PROVIDER: S-EPMC12412100 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature