Dynamic Microstructured Thermoresponsive Interfaces for Label-Free Cell Sorting Based on Nonspecific Interactions.

ABSTRACT: Label-free cell sorting methods and materials are developed in this work. The microstructured thermoresponsive surfaces made of poly(glycidyl methacrylate) (PGMA) and poly(N-isopropylacrylamide-co-glycidyl methacrylate) (PNIPAM-co-GMA) are prepared by phase separation on the submicron scale in thin films and then cross-linked and covalently grafted to the substrate. PGMA domains are used for cell adhesion, while the PNIPAM-co-GMA matrix pushes cells off the surface at a temperature below the lower critical solution temperature (LCST). The microstructure formation and swelling-shrinking caused by changes in temperature are studied experimentally and by using dissipative particle dynamics computer simulations. Experiments with RAW 264.7 murine macrophage-like cells, NIH3

SUBMITTER: Badenhorst R 

PROVIDER: S-EPMC12412105 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature