Advancing synthesis-free and enzyme-free rewritable DNA memory through frameshift encoding and nanopore duplex interruption decoding.

ABSTRACT: DNA data storage is a promising alternative to conventional storage due to high density, low energy consumption, durability, and ease of replication. While information can be encoded into DNA via synthesis, high costs and the lack of rewriting capability limit its applications beyond archival storage. Emerging "hard drive" strategies seek to encode data onto universal DNA templates without de novo synthesis, using methods such as DNA nanostructures and base modifications. However, these approaches face challenges including complexity, low data density, enzymatic constraints, and reliance on costly instrumentation. Here, we introduce a DNA memory system based on frameshift encoding, inspired by viral ribosomal frameshifting, to enable rapid, cost-effective, and parallel data writing on a un

SUBMITTER: Tian K 

PROVIDER: S-EPMC12412213 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature