Microphysiological Uremia Model Reveals Biophysical Potentiators of Vascular Dysfunction.

ABSTRACT: Cardiovascular disease is a leading cause of mortality in individuals with chronic kidney disease (CKD), with hypertension being a major contributor to both kidney damage and increased cardiovascular risk. Despite the established link between CKD and cardiovascular disease, the role of uremic toxins in monocyte-endothelial interactions under hypertensive conditions remains underexplored. Here, a 3D microfluidic model is developed to examine the effects of indoxyl sulfate on monocyte adhesion and extravasation across engineered microvessels embedded in hydrogels with different densities under controlled luminal pressure. Elevated pressure alone in absence of indoxyl sulfate significantly enhanced monocyte adhesion and extravasation, regardless of matrix density, while matrix density regulat

SUBMITTER: Rathod M 

PROVIDER: S-EPMC12412532 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature