Multi-Region Brain Organoids Integrating Cerebral, Mid-Hindbrain, and Endothelial Systems.

ABSTRACT: Brain organoid technology has revolutionized the ability to model human neurodevelopment in vitro. However, current techniques remain limited by their reliance on simplified endothelial cell populations. Multi-Region Brain Organoids (MRBOs) are engineered that integrate cerebral, mid/hindbrain, and complex endothelial organoids into one structure. Unlike earlier approaches based on isolated Human Umbilical Vein Endothelial Cells, the endothelial organoids contain diverse vascular cell types, including progenitors, mature endothelial cells, pericytes, proliferating angiogenic cells, and stromal cells. The strategy employs sequential modulation of key developmental pathways to generate individual organoids, followed by optimized fusion conditions that maintain regional identities while suppo

SUBMITTER: Kshirsagar A 

PROVIDER: S-EPMC12412538 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature