High-Speed Atomic Force Microscopy Reveals the Dynamic Interplay of Membrane Proteins is Lipid-Modulated.

ABSTRACT: The solvent of membrane proteins is the membrane lipids in which they are embedded. Therefore, the nature of the lipids that surround membrane proteins impacts their dynamics and interactions. Unfortunately, how membrane proteins dynamically interact is difficult to study, and little is experimentally known how membrane proteins interplay in a membrane at the molecular scale. Herein, high-speed atomic force microscopy (HS-AFM) is used to dynamically image a well-controlled bottom-up system consisting of two aquaporin-fold membrane proteins, pentameric FocA and tetrameric GlpF, that interact in membranes composed of varying amounts of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and E. coli lipids. It is found that the lipid environment significantly influences membrane protein mo

SUBMITTER: Shin E 

PROVIDER: S-EPMC12412613 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature