Large-Scale Bioprinting of Human Epiblast-Like Models Featuring Disc-Shaped Morphogenesis and Gastrulation Events.

ABSTRACT: Understanding the initial weeks of human development remains challenging due to ethical concerns and the restricted availability of human embryos. Pluripotent stem cell (PSC)-derived epiblast models mimicking gastrulation processes have sparked significant interest in bridging this gap. However, as a newly emerging field, bioengineered models show limited production throughput and complexity in recapitulating epiblasts' disc-shaped morphogenesis. Here, a well-defined laminin/alginate bioink to create epiblast-like models from human induced pluripotent stem cells (hiPSCs) using electro-assisted bioprinting is proposed. This approach enables the generation of large-scale hiPSC-laden microgels that not only facilitate mass transfer but also mimic the structural characteristics of early embryo

SUBMITTER: Luo Y 

PROVIDER: S-EPMC12412627 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature