Individual recombinant repeats of MUC16 display variable binding to CA125 antibodies.

ABSTRACT:

Background

Despite its importance in the clinical management of ovarian cancer, the CA125 biomarker - located on the mucin protein MUC16 - is still not completely understood. Questions remain about MUC16's function and structure, specifically the identity and location of the CA125 epitopes.

Objective

The goal of this study was to characterize the interaction of individual recombinant repeats from the tandem repeat domain of MUC16 with antibodies used in the clinical CA125 II test.

Methods

Using E. coli expression, we isolated nine repeats from the putative antigenic domain of CA125. Amino acid composition of recombinant repeats was confirmed by high-resolution mass spectrometry. We characterized the binding of four antibodies - OC125, M11, "OC125-like," and "M11-like"

SUBMITTER: Wang CW 

PROVIDER: S-EPMC12412824 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature