MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesis.

ABSTRACT: The gene regulatory networks (GRNs) that control early heart formation are beginning to be understood, but lineage-specific GRNs remain largely undefined. We investigated networks controlled by the vital transcription factor MEF2C using a time course of single-nucleus RNA sequencing and ATAC sequencing in wild-type and Mef2c-null embryos. We identified a "posteriorized" cardiac gene signature and chromatin landscape in the absence of MEF2C. Integrating our multiomics data in a deep learning-based model, we constructed developmental trajectories for each of the outflow tract, ventricular, and inflow tract segments and alterations of these in Mef2c-null embryos. We computationally identified segment-specific MEF2C-dependent enhancers with activity in the developing zebrafish he

SUBMITTER: Muncie-Vasic JM 

PROVIDER: S-EPMC12412991 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature