Overcoming target interference in bridging anti-drug antibody (ADA) assay by optimizing sample treatment.

ABSTRACT:

Background

Drug bridging immunoassays are widely employed as the standard approach for detecting anti-drug antibodies (ADAs) in the development of new biological entities. A major challenge in these assays is mitigating target interference, particularly when the soluble target exists in dimeric forms, which can result in false positive signals and compromise assay specificity.

Research design and methods

We developed sensitive and robust ADA assays capable of overcoming target interference to detect antibodies against BI X in both cynomolgus monkey (cyno) plasma and human serum matrices. This was achieved through the implementation of simple sample treatment techniques, specifically, acidification using a panel of different acids, to disrupt dimeric target interactions and m

SUBMITTER: Ye S 

PROVIDER: S-EPMC12413034 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature