A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.

ABSTRACT: Traditional cultivation methods with defined growth media can only isolate and cultivate a small number of microbes. However, much higher microbial diversity has been detected by cultivation-independent tools from a range of natural ecosystems. These represent a large unexplored pool of potentially novel taxa. In this study, a diffusion-based integrative cultivation approach (DICA) was developed to efficiently isolate novel taxonomic candidates from marine sediment. DICA combined a newly designed diffusion-based apparatus called a "microbial aquarium" with modified low-nutrient media. To determine the efficiency of DICA, cultivation results were compared with traditional cultivation approach (TCA). Both cultivation approaches resulted in the isolation of numerous representatives from the p

SUBMITTER: Ahmad T 

PROVIDER: S-EPMC12413390 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature