Molecular treatments to reduce catabolic effects in human meniscus explant models.

ABSTRACT:

Objectives

1. To validate catabolic meniscus explant models induced by cytokines: interleukin-6 ​+ ​interleukin-6 receptor ​+ ​tumor necrosis factor alpha (IL6/TNF) and oncostatin M ​+ ​tumor necrosis factor alpha (OSM/TNF). 2. To evaluate three potential anti-catabolic treatments: i) dexamethasone (DEX), ii) a Link-N peptide (Link-N) and iii) a peptide from chondroadherin (CKF).

Design

Healthy lateral menisci from deceased donors (n ​= ​6; age ​= ​25-70 years, 4 males, 2 females), were sliced and randomized for experimental groups (combinations of the catabolic models and anti-catabolic treatments) and a control group. Culture media were analyzed, every third day until day 18, by mass spectrometry-based proteomics. Linear mixed effect models were used to estimate difference

SUBMITTER: Sjogren A 

PROVIDER: S-EPMC12413724 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature