Electroacupuncture-induced reduction of myocardial ischemia-reperfusion injury via FTO-dependent m6A methylation modulation.

ABSTRACT:

Objective

This study aims to investigate the potential of electroacupuncture to mitigate myocardial ischemia-reperfusion injury (MIRI) by influencing N6-methyladenosine (m6A) methylation through modulation of the fat mass and obesity-associated protein (FTO).

Methods

An experimental murine model of MIRI was established by surgically occluding the left anterior descending coronary artery, followed by reperfusion. Electroacupuncture treatment targeting Neiguan acupoints was administered 7 days before ischemia induction. Cardiac function was evaluated using echocardiography, and myocardial infarction size was assessed through Evans Blue and triphenyltetrazolium chloride dual staining. To measure m6A methylation and mRNA expression of FTO and mediator complex subunit 1 (Med1), R

SUBMITTER: Zou YH 

PROVIDER: S-EPMC12413781 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature