Caffeine Acts as an Agonist of Siglec-6, Inhibits MRGPRX2-Triggered Mast Cell Degranulation and Anaphylactoid Reactions.

ABSTRACT: Background: Mast cells (MCs) are effectors of anaphylactoid reactions. Mas-related G-protein-coupled receptor X2 (MRGPRX2) receptor mediates the direct activation of MCs in anaphylactoid disease. Siglec-6 negatively regulates MC activation and is a promising target in the development of antianaphylactoid reaction drugs. While caffeine exhibits an inhibitory effect against anaphylactic shock, the molecular mechanisms underlying these activities remain unknown. Objectives: Our objective was to investigate the inhibitory effect of caffeine and its underlying molecular mechanism in MRPGRX2-induced MC activation and anaphylactoid reactions. Methods: Local and systemic anaphylactoid reactions in mice and in vitro MC activation experiments were conducted to investigate the ef

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12413947 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature