PI3K Signaling Pathway Inhibitor Affects Myeloma Cells in a Culture-Dependent Manner.

ABSTRACT:

Purpose

The survival and progression of multiple myeloma (MM) cells rely heavily on supportive factors and cells within the MM microenvironment, notably macrophages. The PI3K signaling pathway plays a crucial role in both myeloma cells survival and macrophage polarity, making it a potential target for altering the MM microenvironment dynamics.

Methods

In this study, the impact of LY294002, a PI3K signaling pathway inhibitor, on the viability of U266 myeloma cells in mono-culture and MM patient-derived bone marrow mononuclear cells (BM-MNCs) in co-culture was investigated. Additionally, the effect of treatments on the M1/M2 macrophage ratio was assessed. Cultures were conducted in both two-dimensional (2D) matrix-free and fibrin gel-based three-dimensional (3D) environments.<

SUBMITTER: Janfada M 

PROVIDER: S-EPMC12413969 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature