Differential phagocytosis induces diverse macrophage activation states in malignant gliomas.

ABSTRACT:

Background

Diffuse midline glioma (DMG) and glioblastoma (GBM) are aggressive brain tumors with limited treatment options. Macrophage phagocytosis is a complex, tightly regulated process governed by competing pro-phagocytic and anti-phagocytic signals. CD47-SIRPα signaling inhibits macrophage activity, while radiotherapy (RT) can enhance tumor immunogenicity. How RT and CD47 blockade together modulate macrophage "appetite" and activation states remains poorly understood, particularly in the context of glioma immune evasion and therapy resistance.

Methods

Human and mouse glioma cell lines were exposed to fractionated RT, anti-CD47 monoclonal antibody, or both. Flow cytometry and ELISA quantified the induction of immunogenic cell death (ICD) and expression of damage-associated

SUBMITTER: Lakshmanachetty S 

PROVIDER: S-EPMC12414246 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature