Isolation and identification of a newly discovered broad-spectrum Acinetobacter baumannii phage and therapeutic validation against pan-resistant Acinetobacter baumannii.

ABSTRACT: The treatment of Acinetobacter baumannii (A. baumannii) poses significant clinical challenges due to its multidrug/pan-drug resistance. In this study, we isolated a broad-spectrum lytic A. baumannii phage, named P425, from medical wastewater, targeting nine multidrug-resistant A. baumannii (MDRAB) with diverse capsular types. Biological characterization revealed that P425 maintains activity at pH range of 3-12 and temperature range of 4-50 ​°C. It resists UV irradiation for 20 ​minutes, and had an optimal multiplicity of infection (OMOI) is 0.00001. The adsorption kinetics showed that P425 achieves > 90% within 10 ​minutes of incubation, and the one-step growth curve indicated a 10-min latent period, with a burst size of 184 ​PFU/cell. The genome sequencing results indicated that it harbor

SUBMITTER: Lin M 

PROVIDER: S-EPMC12414385 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature