Scalable Matrigel-Free Suspension Culture for Generating High-Quality Human Liver Ductal Organoids.

ABSTRACT: Liver transplantation is currently the sole definitive treatment option for end-stage liver failure. However, a significant shortage of donors prevails due to high clinical demands. Recently, human liver organoids have shown significant potential in regenerative medicine for liver diseases. Nevertheless, current static cultures of organoids grown in well-plates heavily rely on extracellular matrix hydrogels (Matrigel), thereby limiting both the scalability and quantity of organoid culture. In this study, we present a groundbreaking culture mode that eliminates all reliance on extracellular matrix hydrogels, enabling the successful preparation of functional human liver ductal organoids (LDOs) based on the cell suspension culture mode in a mechanically stirred bioreactor. Initially, the deve

SUBMITTER: Gong S 

PROVIDER: S-EPMC12414634 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature