Analytical validation of a flow cytometric method for the detection and quantification of canine mast cells in peripheral blood, bone marrow, and lymph node.

ABSTRACT: Mast cell tumors (MCTs) are the most common skin neoplasms in dogs and exhibit highly variable biological behavior. Metastasis primarily affects the lymph nodes, though less frequently, MCTs can infiltrate the spleen, liver, peripheral blood, and bone marrow. Flow cytometry of fine needle aspirate samples represents a non-invasive diagnostic procedure that has shown promise for detecting and quantifying mast cells in primary tumors and lymph nodes. However, analytical validation of this method for clinical use is lacking. This study aimed to evaluate the analytical performance of a flow cytometric panel for quantifying mast cells in peripheral blood, bone marrow, and lymph node aspirates from dogs. Key parameters as the limit of blank (LOB), lower limit of detection (LLoD), lower limit of

SUBMITTER: Iamone G 

PROVIDER: S-EPMC12414779 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature