Berberine Sensitises Breast Cancer Cells to Radiation via the Attenuation of DNA Ligase III.

ABSTRACT: Berberine (BBR) is an isoquinoline alkaloid with a variety of biological activities, including anti-microbial and anti-tumoral activities. However, the cellular targets of BBR and the roles of BBR in the radiosensitivity of breast cancer cells are not well defined. In this study, we investigated the effects of BBR on the radiosensitivity of BT549 triple-negative breast cancer cells. Through RNA-seq results, we found that BBR significantly down-regulated the expression of DNA ligases. The results of western blot and comet assay confirmed that BBR attenuated DNA ligase III (LIGIII) expression and caused DNA damage in a dose-dependent manner. The results of electron microscopy showed that BBR decreased mitochondrial copies and induced mitochondrial autophagy. Clonal formation analysis showed

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12414796 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature