First-in-class inhibitors of SbnA reduce siderophore production in Staphylococcus aureus.

ABSTRACT: Siderophore production, along with heme scavenging by hemophores, is one of the main mechanisms exploited by bacteria to achieve an adequate iron supply. Staphylococcus aureus produces two main siderophores, staphyloferrin A (SA) and staphyloferrin B (SB), with the latter produced only by the most invasive, coagulase-positive S. aureus strains. Along the seven steps of the SB biosynthetic pathway, N-(2-amino-2-carboxyethyl)-l-glutamate synthase (SbnA) catalyzes the crucial formation of the intermediate N-(2-amino-2-carboxyethyl)-l-glutamate from O-phospho-L-serine and glutamate. Our functional characterization of the enzyme highlighted that citrate inhibits SbnA with an inhibitory constant (K_i) in the order of magnitude of the physiological concentration of the metabolite. We se

SUBMITTER: Hijazi S 

PROVIDER: S-EPMC12414870 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature