Pathogenicity and whole-genome analysis of a <i>Siniperca chuatsi</i>-derived <i>Nocardia seriolae</i> strain.

ABSTRACT: A bacterial strain (No. 20230510) was isolated from the kidneys of diseased Siniperca chuatsi in Guangxi, China, since 2023. Artificial infection experiments demonstrated that this strain caused the observed disease in S. chuatsi. The isolate underwent morphological, pathological, genomic (whole-genome sequencing, WGS), and antibiotic susceptibility analyses. Infection trials revealed 100% mortality in high-concentration groups, with an LD50 of 3.89 × 104 CFU/mL, indicating high virulence. WGS results showed a circular chromosome of 8,123,106 bp (GC content: 68.14%), containing 7,638 CDSs, 72 tRNAs, and 12 rRNAs. Phylogenomic analysis revealed that strain 20230510 (CP130742) clusters with three N. seriolae strains with 98% bootstrap supporting, confirming its identific

SUBMITTER: Chen L 

PROVIDER: S-EPMC12414942 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature