STAGE: A compact and versatile TnpB-based genome editing toolkit for <i>Streptomyces</i>.

ABSTRACT: Streptomyces are naturally endowed with the capacity to produce a wide array of natural products with biomedical and biotechnological value. They have garnered great interest in synthetic biology applications given the abundance of uncharacterized biosynthetic gene clusters (BGCs). However, progress has been hindered by the limited availability of genetic tools for manipulating these bacteria. Several representative CRISPR-Cas systems have been established in Streptomyces to streamline experimental workflows and improve editing efficiency. Nevertheless, their broader applicability has been constrained by issues such as nuclease activity-related cytotoxicity and the large size of effector proteins. To address these challenges, we present Streptomyces-compatible TnpB-ass

SUBMITTER: Luo J 

PROVIDER: S-EPMC12415229 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature