Color-neutral and reversible tissue transparency enables longitudinal deep-tissue imaging in live mice.

ABSTRACT: Light scattering in biological tissue presents a significant challenge for deep in vivo imaging. Our previous work demonstrated the ability to achieve optical transparency in live mice using intensely absorbing dye molecules, which created transparency in the red spectrum while blocking shorter-wavelength photons. In this paper, we extend this capability to achieve optical transparency across the entire visible spectrum by employing molecules with strong absorption in the ultraviolet spectrum and sharp absorption edges that rapidly decline upon entering the visible spectrum. This color-neutral and reversible tissue transparency method enables optical transparency for imaging commonly used fluorophores in the green and yellow spectra. Notably, this approach facilitates tissue transparency f

SUBMITTER: Keck CHC 

PROVIDER: S-EPMC12415250 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature