MiRNA-24 downregulates KLF6 affecting STAT3 protein expression and phosphorylation regulating melanogenesis in cashmere goat coat.

ABSTRACT:

Objective

This study revealed that miRNA-24 downregulates KLF6, regulating melanogenesis in the coat of Cashmere goats.

Methods

The correlation between miRNA-24 and coat color was determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR). GO/KEGG analysis and bioinformatics tools were used to screen for target genes. Targeted interactions were confirmed using the dual luciferase assay. The expressions of miRNA-24 and KLF6 on different colored skins of Cashmere goats, and the KLF6 expression in B16-F10 cells transfected with miRNA-24 mimics/inhibitors/NC were detected by RT-qPCR, Western Blot, and Immunofluorescence. To investigate the pathway that KLF6 influenced, KLF6 was silenced by RNA interference (RNAi), and the melanogenesis pathway's gene ex

SUBMITTER: Zhang B 

PROVIDER: S-EPMC12415448 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature