A temperature-sensitive mutant screen reveals a translational stress-induced cell-cycle arrest in a thermophilic archaeon.

ABSTRACT: The homology of the archaeal and eukaryotic ribosome along with similarities in their apparatus involved in DNA replication and transcription provide key pieces of evidence underpinning the idea that eukaryotes acquired their core information processing machinery from archaea. Building on this discovery, reverse genetics in archaea has been used to study the functions of many archaeal proteins with eukaryotic homologues. Despite this progress, our general understanding of archaeal growth and division remains unclear, partly because of difficulties of carrying out unbiased genetic screens in archaeal relatives of eukaryotes. Here, we have used a screen of temperature-sensitive mutants in Sulfolobus acidocaldarius to identify regulators of cell growth and division. First, flow cytomet

SUBMITTER: Foo S 

PROVIDER: S-EPMC12415615 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature