CCS-Consensuser: A Haplotype-Aware Consensus Generator for PacBio Amplicon Sequences.

ABSTRACT: DNA sequencing technology has undergone substantial improvements in recent years, to the extent that Third Generation Sequencing platforms are capable of massively generating long-reads. Amplicon sequencing has been among the most popular techniques due to its wide application in diverse fields of biological sciences. However, there is a lack of software specifically designed to analyse intra-individual genetic variation using amplicon long-read data. Here, we present CCS-consensuser, an end-to-end pipeline that generates consensus sequences from amplicon sequencing using high-fidelity reads produced by PacBio circular consensus sequencing (CCS). We evaluated the concordance of the results produced using CCS + CCS-consensuser and other sequencing platforms (Illumina and Sanger), as well as

SUBMITTER: Congrains C 

PROVIDER: S-EPMC12415819 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature