GoldenBraid2.0 <i>E. coli</i>: a comprehensive and characterized toolkit for enterics.

ABSTRACT: Modular cloning systems streamline laboratory workflows by consolidating genetic 'parts' into reusable and modular collections, enabling researchers to fast-track strain construction. The GoldenBraid 2.0 modular cloning system utilizes the cutting property of type IIS restriction enzymes to create defined genetic 'grammars', which facilitate the reuse of standardized genetic parts and assembly of genetic parts in the right order. Here, we present a GoldenBraid 2.0 toolkit of genetic parts designed to accelerate cloning in the model bacterium Escherichia coli. This toolkit features 478 pre-made parts for gene expression and protein tagging as well as strains to expedite cloning and strain construction, enabling researchers to quickly generate functional plasmid-borne or chromosome-in

SUBMITTER: Cooke MB 

PROVIDER: S-EPMC12415853 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature