Library-free data-independent acquisition mass spectrometry enables comprehensive coverage of the cyanobacterial proteome.

ABSTRACT: Cyanobacteria have played a leading role in elucidating the fundamental mechanisms behind oxygenic photosynthesis, carbon fixation, the circadian clock, and phototaxis. Such molecular processes rely on proteins at their core. Thus, proteomics has become an indispensable tool in building our understanding of these processes. Amongst the proteomic approaches used, "shotgun proteomics", where complex protein mixtures are enzymatically digested into peptides and analyzed by liquid chromatography-mass spectrometry, has become the go-to technique for whole-proteome analysis. In this study, we introduce shotgun workflows that excel in speed, throughput, and sensitivity, and allow an in-depth description of the cyanobacterial proteome. The main features of these workflows are the improvement of sa

SUBMITTER: Russo DA 

PROVIDER: S-EPMC12415867 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature