Pinto Bean Supplementation Modulates Gut Microbiota and Improves Markers of Gut Integrity in a Mouse Model of Estrogen Deficiency.

ABSTRACT:

Background

Emerging research suggests that changes in gut microbiota play a key role in menopause-related diseases by modulating gut health.

Objectives

This study investigated the effects of pinto bean (PB) supplementation on gut integrity in an estrogen-deficient mouse model.

Methods

Sixty 3-mo-old female C57BL/6J mice were injected with either sesame oil (vehicle) or vinylcyclohexene diepoxide (VCD, 160 mg/kg) for 30 d to induce estrogen deficiency. Mice were then randomly assigned to 2 dietary groups (n = 15/group): control (AIN-93M) or AIN-93M + 10% (wt/wt) PB for 16 wk. Ovarian failure was confirmed by uterine weight and serum follicle-stimulating hormone (FSH). Gut health was assessed by measuring tight junction proteins, β-glucuronidase activity, short-chain fa

SUBMITTER: Olawale F 

PROVIDER: S-EPMC12415926 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature