Detection of alloreactive T cells from cryopreserved human peripheral blood mononuclear cells.

ABSTRACT: Precise analyses of alloreactive T cell phenotype and function can inform both the nature and intensity of adaptive responses to transplant antigens. However, alloreactive T cells are sparse and difficult to detect, particularly in cryopreserved peripheral blood mononuclear cells (PBMCs) and from hypo-responsive individuals. An assay to identify and phenotype alloreactive cells would be particularly valuable, especially for multi-center clinical trials that often store frozen samples for batch analysis. Herein we demonstrate consistent and reproducible alloreactive T cell detection in cryopreserved PBMC following a short-term mixed lymphocyte reaction (MLR). The inherent background expression levels of activation markers on responder T cells were minimized by including a resting period pri

SUBMITTER: Tanimine N 

PROVIDER: S-EPMC12416544 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature